Endocrine Abstracts

Increased adiposity is associated with insulin resistance, type 2 diabetes and metabolic dysfunction. Altered adipokine secretion, changes in local and systemic glucocorticoid metabolism and increased inflammation have all been postulated as contributory mechanisms. We have previously described changes in subcutaneous adipose tissue (SAT) gene expression that are associated with obesity and glucose intolerance in cross-sectional studies. However, the effects of longitudinal ch...

Introduction: Type 1 iodothyronine deiodinase (DIO1) is one of the three enzymes regulating bioavailability of thyroid hormones. In contrast to DIO2 and DIO3, the specific cellular role of DIO1 remains controversial. Our previous studies showed that DIO1 expression in clear cell renal cell carcinoma (ccRCC) is decreased, followed by lowered intracellular T3 concentration. In this study we explored how re-introduction of DIO1 in ccRCC cells affects their mig...

FSH receptor (FSHR) expression has been shown in gonadal tumours, as well as in endothelial tumour vessel cells of various cancers. FSHR, due to its transmembrane localization could be a good candidate for receptor-mediated targeted cancer therapy. In recent years, a number of membrane disrupting lytic peptides have been used for receptor-based cancer therapy. In the present study, we characterised the specificity and cytotoxicity of a lytic peptide Phor21 conjugated to three ...

Introduction: Molecular testing play increasingly a significant role in pre-operative diagnosis of nodular goiter, as standard methods may not give conclusive decision in choosing optimal treatment approach. BRAF gene mutations are often discovered in the cytological specimens among the patients with papillary thyroid cancer. The aim of the study was to assess the incidence of BRAF T1799A mutation in cellular specimens derived from fine needle aspiration biopsy (FNAB)...

Introduction: The transcription factor 7-like 2 gene (TCF7L2) has the strongest effect on the risk of type 2 diabetes but the mechanism for this is not fully understood. Some genetic variants are associated with pancreatic B-cell function and some single nucleotide polymorphisms show a significant correlation with obesity. The aim of the study was to analyze whether TCF7L2 variants may influence body weight and body fat content, as well as postprandial glucose metabolism in no...

Progesterone (P4) treatment has been shown to have a clear modulating effect on murine Leydig tumor cell (mLTC-1) function, including downregulation of luteinizing hormone receptor. We hypothesized that P4 would stimulate, whereas an antiprogestine mifepristone (MF) block tumor progression in vivo in a transgenic (TG) murine Leydig cell tumor model (inhibin-α promoter-driven SV40 T-antigen (inhα/Tag)) and act similarly on cell proliferation in vitro...

Introduction: Active metabolites of vitamin D have pleiotropic influence on whole body.Aim: The aim of the study was evaluation of the correlation between vitamin D3 and TSH values in population over 65.Materials and methods: 94 patients admitted to diagnosis on Geriatric Department entered the study. People with age above 65 were included to research and divided on two groups according to sex. The material samples were obtained fr...

Introduction: Anthropometric parameters such as: waist circumference, hips circumference, or arm circumference allow to find a correlation between the obesity and the risk of some metabolic disturbances (for example arm circumference index of upper part indicates the level of protein storage in the body).Aim: The aim of the study was to assess the correlation among the arm circumference index, concentration of 25-OH vitamin D3 and lipid profile parameter...

Results of metabolomic studies showed the relation of branched-chain amino acids (BCAA) and aromatic amino acids (AAA) with metabolic disorders.Basing on this observation we assessed amino acid profile in 286 professionally active men (aged 36–60 years). 141 of them met the criteria of metabolic syndrome (MS) and remaining 142 served as controls.Quantitative analysis of BCAA (leucine-LEU, valine-VAL, isoleucine- ILE) and AAA (...

Antiprogestine mifepristone (MF) has been shown to inhibit ovarian epithelial cancer (OEC) cell growth in vitro and in vivo. Recent clinical trials with MF for human OEC were unsuccessful, for unknown reasons. Progesterone (P4) is believed to have preventive measures towards breast, endometrial or hOEC cancers. Hereby we analyzed the effects of P4 and MF on ovarian granulosa cell tumorigenesis (GCT) in vitro and in vivo...